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Bacteria

Wet Spot / Sour Rot

A bacterial contamination common in grain spawn that creates wet, slimy spots. Bacillus bacteria are heat-resistant spore formers that can survive inadequate sterilization. Produces a distinctive sour or fermented smell.

Bacillus spp.High riskDiscard the batch
Wet Spot / Sour Rot in a mushroom grow (illustration)
Reference illustration

What does Wet Spot / Sour Rot look like?

Wet, slimy, translucent spots on grain. Grains appear darker and waterlogged. No visible mycelium growth in affected areas. Grain may clump together.

Color
Brown
Texture
Slimy, wet
Smell
Sour, fermented, acidic
Growth speed
Fast

Why does Wet Spot / Sour Rot show up?

Insufficient sterilization time, overhydrated grain, uneven heating, too-short pressure cooker time.

What do I do if I find Wet Spot / Sour Rot in my grow?

  1. Discard all affected grain.
  2. The bacteria produce endospores that are extremely difficult to eliminate.
  3. Clean pressure cooker thoroughly.
  4. Review grain hydration and sterilization protocol.

How do I prevent Wet Spot / Sour Rot?

  • Sterilize grain at 15 PSI for 90 minutes minimum.
  • Do not overhydrate grain — it should be dry to the touch on the outside.
  • Ensure even heat distribution in pressure cooker.
  • Cool grain fully before inoculation.

At which stage does Wet Spot / Sour Rot appear?

StageRiskWhat happensHow to prevent it
PreparationHighMost common cause — inadequate grain sterilization or overhydration.Follow precise grain prep: soak, simmer, dry, then sterilize at 15 PSI 90+ min.
InoculationHighOften appears within days of inoculation due to surviving spores.Ensure grain is fully cooled before inoculating. Work cleanly.
ColonizationModerateWet spots become obvious as healthy mycelium grows around them.Shake grain jars to check for wet spots. Discard any suspect jars.
FruitingLowRarely an issue at fruiting if spawn was clean.—
HarvestNo risk——

Updated: February 24, 2026

This guide is for orientation. A reliable diagnosis needs a lab. If in doubt, ask an experienced mycologist.

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